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EC 1.14.13.54 is Orphan !
Common name :Ketosteroid monooxygenase

Systematic name :Ketosteroid,NADPH:oxygen oxidoreductase (20-hydroxylating, ester-producing/20-hydroxylating, side-chain cleaving/17-hydroxylating, lactonizing)

Other names :17-alpha-hydroxyprogesterone, NADPH(2):oxygen oxidoreductase (20-hydroxylating, side-chain cleaving)
Androstenedione, NADPH(2):oxygen oxidoreductase (17-hydroxylating, lactonizing)
Progesterone, NADPH(2):oxygen oxidoreductase (20-hydroxylating, ester-producing)
Steroid-ketone monooxygenase
Cofactors : FAD.

Comments :A single FAD-containing enzyme catalyzes three types of monooxygenase (Baeyer-Villiger oxidation) reaction. The oxidative esterification of a number of derivatives of progesterone to produce the corresponding 17-alpha-hydroxysteroid 17-acetate ester, such as testosterone acetate, is shown in Reaction (2). The oxidative lactonization of a number of derivatives of androstenedione to produce the 13,17-secoandrosteno-17,13-alpha-lactone, such as testololactone, is shown in Reaction (3). The oxidative cleavage of the 17-beta-side-chain of 17-alpha-hydroxyprogesterone to produce androstenedione and acetate is shown in Reaction (4). Reaction (2) is also catalyzed by EC 1.14.99.4 (progesterone monooxygenase), and Reactions (3) and (4) correspond to that catalyzed by EC 1.14.99.12 (androst-4-ene-3,17-dione monooxygenase). The possibility that a single enzyme is responsible for the reactions ascribed to EC 1.14.99.4 and EC 1.14.99.12 in other tissues cannot be excluded. A single FAD-containing enzyme catalyzes three types of monooxygenase (Baeyer-Villiger oxidation) reaction. The oxidative esterification of a number of derivatives of progesterone to produce the corresponding 17-alpha-hydroxysteroid 17-acetate ester, such as testosterone acetate, is shown in reaction (2). The oxidative lactonization of a number of derivatives of androstenedione to produce the 13,17-secoandrosteno-17,13-alpha-lactone, such as testololactone, is shown in reaction (3). The oxidative cleavage of the 17-beta-side-chain of 17-alpha-hydroxyprogesterone to produce androstenedione and acetate is shown in reaction (4). reaction (2) is also catalyzed by EC 1.14.99.4 and reactions (3) and (4) correspond to that catalyzed by EC 1.14.99.12. The possibility that a single enzyme is responsible for the reactions ascribed to EC 1.14.99.4 and EC 1.14.99.12 in other tissues cannot be excluded.

Created :EC 1.14.13.54 created 1999

BRENDA organisms :Bacillus megaterium
Neonectria radicicola
Rhodococcus rhodochrous

Swiss-ProtNo protein sequences are associated with EC 1.14.13.54 in Swiss-Prot

TrEMBLNo protein sequences are associated with EC 1.14.13.54 in TrEMBL

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Links to other databases
NC-IUBMB, INTENZ, ENZYME, PDB, BRENDA, KEGG, BIOCYC, PubMed,NCBI-Entrez

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